自ABW&Mogengel基質膠推出以來,在業界贏得了良好的口碑,感謝廣大客戶們的認可!公司將一如既往嚴格把控產品質量,誓為廣大科研工作者提供優質的產品和技術服務!
同時為了感謝和回饋廣大的科研工作者,ABW&MG發起【引用ABW&MG基質膠,領取高額獎金】的長期有效活動,若您使用了ABW&MG產品并發表了文章,請聯系我們領取相應的獎勵哦!
4.12. Transwell Assay
Transwell assays were performed using transwell chambers with or without Matrigel coating to detect cell invasion and migration ability, respectively. For invasion assay, Matrigel (082706, ABW, China) was dissolved with a serum-free medium at 1:8, and 100 µL was added to each upper chamber. Then, 200 µL serum-free medium containing 5 × 104 was added to the upper chamber, while the lower chamber was added with 600 µL 10% FBS medium. The cells were fixed and stained after 24 h of incubation. For migration assay, 200 µL serum-free medium containing 2.5 × 104 was added to the upper chamber, while the lower chamber was added with 800 µL 10% FBS medium. The next procedures are the same as for the invasion assay. Three randomly selected fields of view were photographed under the microscope and the images were counted using ImageJ software (National Institutes of Health).
聯系我們
廈門?;锟萍加邢薰?/span> 公司地址:廈門火炬高新區(翔安)產業區翔星路88號育成中 心E205-234室 技術支持:化工儀器網掃一掃 更多精彩
?;娞柖S碼
?;曨l號二維碼